个人基本信息
姓名：牛康乐性别：男
职称：讲师
邮编：450002
通讯地址：河南省郑州市金水区平安大道218号，河南农业大学生命科学学院
E-mail：kangleniu163@163.com
个人简介
2025.09-至今 河南农业大学 生命科学学院 微生物学系 讲师
2020.12-2025.01 山东大学 博士后
2016.09-2020.12 山东大学 微生物技术国家重点实验室 微生物学专业 博士学位
2013.09-2016.06 山东大学 生命科学学院 生物工程专业 硕士学位
2009.09-2013.07 河南理工大学 资源环境学院 生物技术专业 学士学位
2020.12-2025.01 山东大学 博士后
2016.09-2020.12 山东大学 微生物技术国家重点实验室 微生物学专业 博士学位
2013.09-2016.06 山东大学 生命科学学院 生物工程专业 硕士学位
2009.09-2013.07 河南理工大学 资源环境学院 生物技术专业 学士学位
教授课程
《微生物学》、《酶工程》、《生物信息学》
主要研究领域
1、利用人工智能、酶工程及合成生物学技术，开发新型生物催化剂和高效细胞工厂，实现生物秸秆的高值化利用及高值产品的零碳智造；
2、新型酶分子的挖掘、定向改造及催化机制解析；
3、酶分子热稳定性的分子机制解析与功能优化。
2、新型酶分子的挖掘、定向改造及催化机制解析；
3、酶分子热稳定性的分子机制解析与功能优化。
发表论著
论文：
1. Kangle Niu, Ziyuan Ni, Shiyu Yuan, Zixian Wang, Xu Fang*. One-pot synthesis of γ-cyclodextrin of high purity from non-food cellulose via an in vitro ATP-free synthetic enzymatic biosystem[J]. Carbohydrate Polymers, 2025, 347: 122735.
2. Kangle Niu, Liming Yan, Ji Ge, Ziyuan Ni, Zhengyao Liu, Qinghua Liu, Zhenzhen Wang, Zhiqiang Du, Zhihong Wu, Zhiyong Lou, Xu Fang*. Artificial trimerization of β-glucosidase for enhanced thermostability and activity via computational redesign[J]. International Journal of Biological Macromolecules, 2025, 286: 138275.
3. Kangle Niu, Ziyuan Ni, Zixian Wang, Lijuan Han, Lulu Liu, Wei Ma, Zhengyao Liu, Laichuang Han, Meijuan Shi, Lihua Zhang, Zhihong Wu, Xu Fang*. Engineering region flexibility of cellobiohydrolase Ⅰ for efficient hydrolysis of cellulose based on molecular dynamics simulation[J]. Industrial Crops & Products, 2024, 222: 119707.
4. Kangle Niu, Zhengyao Liu, Yuhui Feng, Tianlong Gao, Zhenzhen Wang, Piaopiao Zhang, Zhiqiang Du, Daming Gao, Xu Fang*. A novel strategy for efficient disaccharides synthesis from glucose by β-glucosidase[J]. Bioresources and bioprocessing, 2020, 7: 45.
5. Lijuan Han, Zixian Wang, Shiyu Yuan, Kangle Niu, Zhihong Wu, Xu Fang*. Upgraded multienzymes activities by Trichoderma reesei secretion using mixed Avicel and wheat bran as inducing carbon source[J]. Biocatalysis and Agricultural Biotechnology, 2025, 66, 103610.
6. Daming Liu, Zixian Wang, Shiyu Yuan, Ziyuan Ni, Kangle Niu, Zhihong Wu, Xu Fang*. One-pot coproduction of high-value-added γ-cyclodextrin and myo-inositol from nonfood cellulose using an in vitro ATP-free enzymatic biosystem[J]. ACS Sustainable Chemistry & Engineering, 2025, 13(24)，9132-9137.
7. Wei Ma, Fengyi Li, Longyue Li, Bin Li, Kangle Niu, Qinghua Liu, Laichuang Han, Lijuan Han, Xu Fang*. Production of D-tagatose, bioethanol, and microbial protein from the dairy industry by-product whey powder using an integrated bioprocess[J]. Biotechnology Journal, 2024, 19(2), 2300415.
8. Fengyi Li, Wei Ma, Lulu Liu, Kangle Niu, Daming Liu, Wencheng Yin, Xudong Zhang, Lijuan Han*, Xu Fang*. Reprogramming the Metabolic Network in Kluyveromyces lactis with a transcriptional switch for de novo lacto-N-biose synthesis[J]. Journal of Agricultural and Food Chemistry, 2023, 71(23), 9031-9039.
9. Zhiqiang Du, Fengyi Li, Zhengyao Liu, Yinshuang Tan, Kangle Niu, Xu Fang*. Driving an in vitro multienzymatic cascade of laminaribiose biosynthesis from non-food cellulose with balancing the precursor supply[J]. Industrial Crops and Products, 2022, 182, 114878.
10. Lijuan Han, Baojie Jiang, Wei Wang, Gaosheng Wang, Yinshuang Tan, Kangle Niu, Xu Fang*. Alleviating nonproductive adsorption of lignin on CBM through the addition of cationic additives for lignocellulosic hydrolysis[J]. ACS Applied Bio Materials, 2022, 5(5), 2253-2261.
11. Zhiqiang Du, Zhengyao Liu, Yinshuang Tan, Kangle Niu, Wei Guo, Yangyang Jia, Xu Fang*. Lacto-N-biose synthesis via a modular enzymatic cascade with ATP regeneration[J]. Iscience, 2021, 24(3)102236.
12. Lara Sindhu, Kangle Niu, Xiaolin Liu, Shou-Qing Ni, Xu Fang*. Effect of Fe2+ addition on anammox consortia, nitrogen removal performance and functional genes analysis during start-up of anammox process[J]. Journal of Water Process Engineering, 2021, 43: 102251.
13. Lijuan Han, Kuimei Liu, Wei Ma, Yi Jiang, Shaoli hou, Yinshuang Tan, Quanquan Yuan, Kangle Niu, Xu Fang*. Redesigning transcription factor Cre1 for alleviating carbon catabolite repression in Trichoderma reesei[J]. Synthetic and systems biotechnology, 2020, 5(3): 230-235.
14. Lijuan Han, Yinshuang Tan , We Ma, Kangle Niu, Shaoli Hou, Wei Guo, Yucui Liu, Xu Fang*. Precision engineering of the transcription factor Cre1 in Hypocrea jecorina (Trichoderma reesei) for efficient cellulase production in the presence of glucose[J]. Frontiers in Bioengineering and Biotechnology, 2020, 8: 852.
15. Yi Jiang, Yu Shen, Lichuan Gu, Zhenzhen Wang, Ning Su, Kangle Niu, Wei Guo, Shaoli Hou, Xiaoming Bao, Chaoguang Tian, Xu Fang*. Identification and Characterization of an Efficient D-Xylose Transporter in Saccharomyces cerevisiae[J]. Journal of Agricultural and Food Chemistry, 2020, 68(9): 2702-2710.
16. Fangzhong Wang, Mingyu Wang, Qi Zhao, Kangle Niu, Shasha Liu, Didi He, Yan Liu, Shiping Xu, Xu Fang*. Exploring the relationship between Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17[J]. Frontiers in Microbiology, 2019, 10: 2035.
17. Ruiqin Zhang, Yucui Liu, Yi Zhang, Dan Feng, Shaoli Hou, Wei Guo, Kangle Niu, Yi Jiang, Lijuan Han, Lara Sindhu, Xu Fang*. Identification of a thermostable fungal lytic polysaccharide monooxygenase and evaluation of its effect on lignocellulosic degradation[J]. Applied microbiology and biotechnology, 2019: 14(103):1-12.
18. Fangzhong Wang, Ruiqin Zhang, Lijuan Han, Wei Guo, Zhiqiang Du, Kangle Niu, Yucui Liu, Chunjiang Jia, Xu Fang*. Use of fusion transcription factors to reprogram cellulase transcription and enable efficient cellulase production in Trichoderma reesei[J]. Biotechnology for biofuels, 2019, 12(1): 1-12.
19. Mingyu Wang, Meiling Zhang, Ling Li, Yanmei Dong, Kuimei Liu, Ruiqin Zhang, Baojie Zhang, Kangle Niu, Xu Fang*. Role of Trichoderma reesei mitogen-activated protein kinases (MAPKs) in cellulase formation[J]. Biotechnology for biofuels, 2017, 10(1): 99.
20. Fangzhong Wang, Yi Jiang, Wei Guo, Kangle Niu，Ruiqing Zhang, Shaoli Hou, Mingyu Wang, Yong Yi, Changxiong Zhu, Chunjiang Jia, Xu Fang*. An environmentally friendly and productive process for bioethanol production from potato waste[J]. Biotechnology for biofuels, 2016, 9(1): 50.
21. Baojie Jiang, Ruiqin Zhang, Dan Feng, Fangzhong Wang, Kuimei Liu, Yi Jiang, Kangle Niu, Quanquan Yuan, Mingyu Wang, Hailong Wang, Youming Zhang, Xu Fang*. A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum[J]. Frontiers in Microbiology, 2016, 7: 485.
22. Mingyu Wang, Qi Zhao, Ling Li, Kangle Niu, Yi Li, Fangzhong Wang, Baojie Jiang, Kuimei Liu, Yi Jiang, Xu Fang*. Contributing factors in the improvement of cellulosic H2 production in Clostridium thermocellum/Thermoanaerobacterium co-cultures[J]. Applied Microbiology and Biotechnology, 2016, 100(19): 8607-8620.
著作：
《Fungal Cellulolytic Enzymes: Microbial Production and Application》，Springer Singapore出版社，学术参考著作，2018年8月，参编。
1. Kangle Niu, Ziyuan Ni, Shiyu Yuan, Zixian Wang, Xu Fang*. One-pot synthesis of γ-cyclodextrin of high purity from non-food cellulose via an in vitro ATP-free synthetic enzymatic biosystem[J]. Carbohydrate Polymers, 2025, 347: 122735.
2. Kangle Niu, Liming Yan, Ji Ge, Ziyuan Ni, Zhengyao Liu, Qinghua Liu, Zhenzhen Wang, Zhiqiang Du, Zhihong Wu, Zhiyong Lou, Xu Fang*. Artificial trimerization of β-glucosidase for enhanced thermostability and activity via computational redesign[J]. International Journal of Biological Macromolecules, 2025, 286: 138275.
3. Kangle Niu, Ziyuan Ni, Zixian Wang, Lijuan Han, Lulu Liu, Wei Ma, Zhengyao Liu, Laichuang Han, Meijuan Shi, Lihua Zhang, Zhihong Wu, Xu Fang*. Engineering region flexibility of cellobiohydrolase Ⅰ for efficient hydrolysis of cellulose based on molecular dynamics simulation[J]. Industrial Crops & Products, 2024, 222: 119707.
4. Kangle Niu, Zhengyao Liu, Yuhui Feng, Tianlong Gao, Zhenzhen Wang, Piaopiao Zhang, Zhiqiang Du, Daming Gao, Xu Fang*. A novel strategy for efficient disaccharides synthesis from glucose by β-glucosidase[J]. Bioresources and bioprocessing, 2020, 7: 45.
5. Lijuan Han, Zixian Wang, Shiyu Yuan, Kangle Niu, Zhihong Wu, Xu Fang*. Upgraded multienzymes activities by Trichoderma reesei secretion using mixed Avicel and wheat bran as inducing carbon source[J]. Biocatalysis and Agricultural Biotechnology, 2025, 66, 103610.
6. Daming Liu, Zixian Wang, Shiyu Yuan, Ziyuan Ni, Kangle Niu, Zhihong Wu, Xu Fang*. One-pot coproduction of high-value-added γ-cyclodextrin and myo-inositol from nonfood cellulose using an in vitro ATP-free enzymatic biosystem[J]. ACS Sustainable Chemistry & Engineering, 2025, 13(24)，9132-9137.
7. Wei Ma, Fengyi Li, Longyue Li, Bin Li, Kangle Niu, Qinghua Liu, Laichuang Han, Lijuan Han, Xu Fang*. Production of D-tagatose, bioethanol, and microbial protein from the dairy industry by-product whey powder using an integrated bioprocess[J]. Biotechnology Journal, 2024, 19(2), 2300415.
8. Fengyi Li, Wei Ma, Lulu Liu, Kangle Niu, Daming Liu, Wencheng Yin, Xudong Zhang, Lijuan Han*, Xu Fang*. Reprogramming the Metabolic Network in Kluyveromyces lactis with a transcriptional switch for de novo lacto-N-biose synthesis[J]. Journal of Agricultural and Food Chemistry, 2023, 71(23), 9031-9039.
9. Zhiqiang Du, Fengyi Li, Zhengyao Liu, Yinshuang Tan, Kangle Niu, Xu Fang*. Driving an in vitro multienzymatic cascade of laminaribiose biosynthesis from non-food cellulose with balancing the precursor supply[J]. Industrial Crops and Products, 2022, 182, 114878.
10. Lijuan Han, Baojie Jiang, Wei Wang, Gaosheng Wang, Yinshuang Tan, Kangle Niu, Xu Fang*. Alleviating nonproductive adsorption of lignin on CBM through the addition of cationic additives for lignocellulosic hydrolysis[J]. ACS Applied Bio Materials, 2022, 5(5), 2253-2261.
11. Zhiqiang Du, Zhengyao Liu, Yinshuang Tan, Kangle Niu, Wei Guo, Yangyang Jia, Xu Fang*. Lacto-N-biose synthesis via a modular enzymatic cascade with ATP regeneration[J]. Iscience, 2021, 24(3)102236.
12. Lara Sindhu, Kangle Niu, Xiaolin Liu, Shou-Qing Ni, Xu Fang*. Effect of Fe2+ addition on anammox consortia, nitrogen removal performance and functional genes analysis during start-up of anammox process[J]. Journal of Water Process Engineering, 2021, 43: 102251.
13. Lijuan Han, Kuimei Liu, Wei Ma, Yi Jiang, Shaoli hou, Yinshuang Tan, Quanquan Yuan, Kangle Niu, Xu Fang*. Redesigning transcription factor Cre1 for alleviating carbon catabolite repression in Trichoderma reesei[J]. Synthetic and systems biotechnology, 2020, 5(3): 230-235.
14. Lijuan Han, Yinshuang Tan , We Ma, Kangle Niu, Shaoli Hou, Wei Guo, Yucui Liu, Xu Fang*. Precision engineering of the transcription factor Cre1 in Hypocrea jecorina (Trichoderma reesei) for efficient cellulase production in the presence of glucose[J]. Frontiers in Bioengineering and Biotechnology, 2020, 8: 852.
15. Yi Jiang, Yu Shen, Lichuan Gu, Zhenzhen Wang, Ning Su, Kangle Niu, Wei Guo, Shaoli Hou, Xiaoming Bao, Chaoguang Tian, Xu Fang*. Identification and Characterization of an Efficient D-Xylose Transporter in Saccharomyces cerevisiae[J]. Journal of Agricultural and Food Chemistry, 2020, 68(9): 2702-2710.
16. Fangzhong Wang, Mingyu Wang, Qi Zhao, Kangle Niu, Shasha Liu, Didi He, Yan Liu, Shiping Xu, Xu Fang*. Exploring the relationship between Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17[J]. Frontiers in Microbiology, 2019, 10: 2035.
17. Ruiqin Zhang, Yucui Liu, Yi Zhang, Dan Feng, Shaoli Hou, Wei Guo, Kangle Niu, Yi Jiang, Lijuan Han, Lara Sindhu, Xu Fang*. Identification of a thermostable fungal lytic polysaccharide monooxygenase and evaluation of its effect on lignocellulosic degradation[J]. Applied microbiology and biotechnology, 2019: 14(103):1-12.
18. Fangzhong Wang, Ruiqin Zhang, Lijuan Han, Wei Guo, Zhiqiang Du, Kangle Niu, Yucui Liu, Chunjiang Jia, Xu Fang*. Use of fusion transcription factors to reprogram cellulase transcription and enable efficient cellulase production in Trichoderma reesei[J]. Biotechnology for biofuels, 2019, 12(1): 1-12.
19. Mingyu Wang, Meiling Zhang, Ling Li, Yanmei Dong, Kuimei Liu, Ruiqin Zhang, Baojie Zhang, Kangle Niu, Xu Fang*. Role of Trichoderma reesei mitogen-activated protein kinases (MAPKs) in cellulase formation[J]. Biotechnology for biofuels, 2017, 10(1): 99.
20. Fangzhong Wang, Yi Jiang, Wei Guo, Kangle Niu，Ruiqing Zhang, Shaoli Hou, Mingyu Wang, Yong Yi, Changxiong Zhu, Chunjiang Jia, Xu Fang*. An environmentally friendly and productive process for bioethanol production from potato waste[J]. Biotechnology for biofuels, 2016, 9(1): 50.
21. Baojie Jiang, Ruiqin Zhang, Dan Feng, Fangzhong Wang, Kuimei Liu, Yi Jiang, Kangle Niu, Quanquan Yuan, Mingyu Wang, Hailong Wang, Youming Zhang, Xu Fang*. A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum[J]. Frontiers in Microbiology, 2016, 7: 485.
22. Mingyu Wang, Qi Zhao, Ling Li, Kangle Niu, Yi Li, Fangzhong Wang, Baojie Jiang, Kuimei Liu, Yi Jiang, Xu Fang*. Contributing factors in the improvement of cellulosic H2 production in Clostridium thermocellum/Thermoanaerobacterium co-cultures[J]. Applied Microbiology and Biotechnology, 2016, 100(19): 8607-8620.
著作：
《Fungal Cellulolytic Enzymes: Microbial Production and Application》，Springer Singapore出版社，学术参考著作，2018年8月，参编。
获得主要荣誉及科研成果
科研项目
1. 山东省自然科学基金青年基金，新型逆水解活性葡萄糖苷酶的蛋白质工程及应用，2025.1-2027.12，主持。
2. 青岛市博士后应用研究项目，葡萄糖苷酶的挖掘改造及其底物识别机制解析，2022.1-2023.12，主持。
3. 国家自然科学基金项目，AA9家族裂解多糖单加氧酶（LPMOs）协同降解纤维素的分子机制及其蛋白质工程，2023.01-2026.12，参与。
4. 国家重点研发计划项目，非细胞生物合成系统的构建与应用，2019.07-2024.06，参与。
5. 国家重点研发计划项目，农业秸秆高效预处理及酶解关键技术，2019.04-2023.03，参与。
6. 国家自然科学基金项目，真菌纤维素酶与木质素非产出性吸附的分子机理研究及纤维素酶的改造，2019.01-2022.12，参与。
国家发明专利
1. 方诩，候少莉，刘莎莎，牛康乐，一种用甘薯废弃物制备糖和乙醇的方法，专利授权号：ZL 201310384161.0
2. 方诩，牛康乐，韩丽娟，王明钰，一种改造的β-葡萄糖苷酶及其表达基因与应用，专利授权号：ZL 201410438150.0
3. 方诩，候少莉，刘莎莎，牛康乐，一种用甘薯废弃物制备糖和乙醇的方法，专利申请号：CN 201310384161.0
4. 方诩，牛康乐，韩丽娟，王明钰，一种改造的β-葡萄糖苷酶及其表达基因与应用，专利申请号：CN 201410438150.0
5. 方诩，韩丽娟，牛康乐，吴志红，谭银霜。一种丝状真菌重组菌株在纤维素酶生产领域的应用，专利申请号CN 2021110492981
6. 方诩，牛康乐，倪紫源，马维，吴志红，一种来自里氏木霉的纤维素二糖水解酶的突变体及其应用，专利申请号：CN 2023116714585
7. 方诩，牛康乐，一种具有高比酶活性和高热稳定性的β-葡萄糖苷酶及其制备方法和应用，专利申请号CN 201910910909.3
8. 方诩，牛康乐，倪紫源；一种生物合成γ-环糊精的方法及其应用，专利申请号： CN 202410121570X
1. 山东省自然科学基金青年基金，新型逆水解活性葡萄糖苷酶的蛋白质工程及应用，2025.1-2027.12，主持。
2. 青岛市博士后应用研究项目，葡萄糖苷酶的挖掘改造及其底物识别机制解析，2022.1-2023.12，主持。
3. 国家自然科学基金项目，AA9家族裂解多糖单加氧酶（LPMOs）协同降解纤维素的分子机制及其蛋白质工程，2023.01-2026.12，参与。
4. 国家重点研发计划项目，非细胞生物合成系统的构建与应用，2019.07-2024.06，参与。
5. 国家重点研发计划项目，农业秸秆高效预处理及酶解关键技术，2019.04-2023.03，参与。
6. 国家自然科学基金项目，真菌纤维素酶与木质素非产出性吸附的分子机理研究及纤维素酶的改造，2019.01-2022.12，参与。
国家发明专利
1. 方诩，候少莉，刘莎莎，牛康乐，一种用甘薯废弃物制备糖和乙醇的方法，专利授权号：ZL 201310384161.0
2. 方诩，牛康乐，韩丽娟，王明钰，一种改造的β-葡萄糖苷酶及其表达基因与应用，专利授权号：ZL 201410438150.0
3. 方诩，候少莉，刘莎莎，牛康乐，一种用甘薯废弃物制备糖和乙醇的方法，专利申请号：CN 201310384161.0
4. 方诩，牛康乐，韩丽娟，王明钰，一种改造的β-葡萄糖苷酶及其表达基因与应用，专利申请号：CN 201410438150.0
5. 方诩，韩丽娟，牛康乐，吴志红，谭银霜。一种丝状真菌重组菌株在纤维素酶生产领域的应用，专利申请号CN 2021110492981
6. 方诩，牛康乐，倪紫源，马维，吴志红，一种来自里氏木霉的纤维素二糖水解酶的突变体及其应用，专利申请号：CN 2023116714585
7. 方诩，牛康乐，一种具有高比酶活性和高热稳定性的β-葡萄糖苷酶及其制备方法和应用，专利申请号CN 201910910909.3
8. 方诩，牛康乐，倪紫源；一种生物合成γ-环糊精的方法及其应用，专利申请号： CN 202410121570X
获奖情况
1.中国食品科学技术学会科学技术奖二等奖，2024年。
2.中国循环经济协会科学技术奖二等奖，2018年。
3.第四届全国微生物学研究生博士后学术论坛优秀报告奖，2015年。
4.诺维信杯酶在食品工业中的应用创意大赛优秀奖，2014年。
1.中国食品科学技术学会科学技术奖二等奖，2024年。
2.中国循环经济协会科学技术奖二等奖，2018年。
3.第四届全国微生物学研究生博士后学术论坛优秀报告奖，2015年。
4.诺维信杯酶在食品工业中的应用创意大赛优秀奖，2014年。
